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Extra resources for Antibiotics - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References
Studies 21 Timing: Fiscal Year 2001; Project Start 30-SEP-1985; Project End 31-MAY-2006 Summary: (provided by applicant): The incidence of antibiotic resistance is increasing in many groups of disease-causing bacteria. Although some bacteria become resistant to antibiotics through mutation, acquisition of antibiotic resistance genes from other bacteria is probably more common. This proposal focuses on the genus Bacteroides. Bacteroides species are among the numerically predominant species of bacteria in the human colon.
45 mega-Dalton), which associate upon the initiation of protein biosynthesis. The immediate objectives of this proposal are (a) to use the high-resolution structures of the two eubacterial ribosomal subunits determined by us as references for the elucidation of the structures of ribosomal complexes with substrate analogs, functional ligands, inhibitors and antibiotics; (b) to exploit these structures for illuminating the mechanisms involved in peptide bond formation, decoding, translocation, inhibition by and resistance to antibiotics; (c) to progress toward high resolution structure determination of complexes capturing the whole ribosome at defined functional states; (d) to advance towards the determination of the structure of eukaryotic ribosomes.
We will characterize the ability of HD5 transgenic mice, compared to control wild-type mice, to resist enteric infection by Salmonella typhimurium (Aim 1A), and parallel experiments will extend to other enteric pathogens (Aim 1B). We will examine the impact of transgenic HD5 expression on resident microflora of the mouse intestine (Aim 1C). The antimicrobial activity contributed by transgenic expression will be quantitated in vitro, including analysis of isolated crypts (Aim 1D). The HD6 gene and peptide share little sequence identity to HD5, yet they are expressed together in Paneth cells.