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Primary DNA lesions are detectable by adduct-specific antibodies and can be measured by quantitative immuno-fluorescence analysis (lCA; shaded area); repair intermediates can be recognized by the comet assay (boxed area). The inhibition of distinct steps by three repair modifiers is indicated (06-benzylguanine inactivates the alkyltransferase AT; methoxyamine prevents DNA incision by AP-endonuclease(s); aphidicoline inhibits gap filling by DNA polymerases) profiles or to the therapeutic response.
2. 3. 4. 5. 6. 7. 8. 9. 10. Baurmann H, Cherif D, Berger R (1993) Interphase cytogenetics by fluorescent in situ hybridization (FISH) for characterization of monosomy-7-associated myeloid disorders. Leukemia 7: 384 Feuring-Buske M, Haase D, Konemann S, Troff C,Hiddemann W, Wormann B (1995) Trisomy 4 in the stem cell compartment of acute myeloid leukemia. A case report. Leukemia: 1318-1320 Feuring-Buske M, Haase D, Konemann S, Troff C, Verbeek W, Hiddemann W, Wormann B (1997) Trisomy 4 in "stem cell like" leukemic cells of a patient with AML.
Quantification of Fluorescence Signals in the Nuclear DNA of Individual Cells (lCA). ) , 14 (for TRITC) and 15 (for ethidium bromide) were used for the visualization of fluorescence of the individual cells. Fluorescence signals were amplified and recorded by a dual mode CCD camera (Photonics, Hamamatsu City, Japan), and fed into a four parameter image analysis program (ACAS; Ahrens, Bargteheide, Germany). This program performs integration of fluorescence images with low signal to noise ratios. Thresholds were set to discriminate background from DNA staining signals and to determine the image points to be included in the evaluation.